SHORT TALKS
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MONDAY
12:30 A. Life at Low Temperature I
SL1A. Effect of Ice Nucleation Temperature on the Survival of Excised Tissue from the Alpine Cockroach Celatoblatta quinquemaculata M. R Worland and D. A.Wharton British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road., Cambridge CB3 0ET, United Kingdom; Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand mrwo@bas.ac.uk
SL2A. Development and Results of Quality Assurance Testing for Mass-Reared and Laboratory-Colonized Insects After Embryo Cryopreservation. R. A. Leopold, A. Rajamohan and T. E. Shelly USDA/ARS Biosciences Research Laboratory, Fargo, ND; North Dakota State University, Department of Entomology, Fargo, ND; USDA/APHIS Plant Protection Research Laboratory, Waimanalo, HI, U.S.A. leopoldr@fargo.ars.usda.gov
B. Life at Low Temperature II
SL1B. Rapid Cold Hardening in the Antarctic Springtail Cryptopygus Antarcticus M. R. Worland and P. Convey British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road., Cambridge CB3 0ET, United Kingdom
SL2B. Hemolymph Antifreeze Proteins from the Longhorn Beetle Rhagium inquisitor Erlend Kristiansen, Hans Ramløv, Lars Hagen and Karl Erik Zachariassen Department of Biology & Department of cancer research and molecular medicine, Norwegian University of Science and Technology (NTNU), Norway Department of Life Sciences and Chemistry, Roskilde University, Denmark erlend.kristiansen@bio.ntnu.no
17:00 A. Cryopreservation of Plants and Vegetal Cells
SL3A. Thermal Analysis of Water Status in Plants at Low Temperatures J. Zamecnik, A. Bilavcik, M. Faltus and J. Sestak Department of Molecular Biology, Research Institute of Crop Production, Drnovska 507, CZ 161 06 Prague 6, Czech Republic zamecnik@vurv.cz
SL4A. Response of Cytoskeletal Elements of Cassava Callus to Cryostorage D. J. Mycock School of Animal, Plant & Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa Dave@biology.biol.wits.ac.za
SL5A. Non-Colligative Role of Sucrose in Cryopreservation of Cacao (Theobroma Cacao L.) Somatic Embryos J.-Y. Fang, M. Sacandé, A. Wetten and H. Pritchard School of Plant Sciences, University of Reading, Reading, Berkshire RG6 6AS (United Kingdom); Seed Conservation Department, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, Haywards Heath, West Sussex RH17 6TN, United Kingdom
SL6A. Cryopreservation of Eukaryotic Soil Algae A. Lukešová, M. R. Worland and P. Hrouzek Institute of Soil Biology, Ceske Budejovice, Czech Republic
SL7A. The Importance of Cryopreservation: The Acoi Collection Case R. Amaral, H. Osório, C. Mendes, V. Ramos, F. Santos and L. Santos Departamento de Botânica da Universidade de Coimbra, Calçada Martim de Freitas 3000 Coimbra, Portugal liliamas@ci.uc.pt
B. Mechanisms of Adaptation of Microorganisms to Low Temperature and Cryopreservation of Microorganisms
SL3B. Interspecies Protection Against Freezing Stress Within a Microbial Community E. Denou, M. Guéguen and J.-M. Panoff Laboratoire de Microbiologie Alimentaire (UA 3213 – USC INRA) – IBFA, Université de Caen Basse-Normandie, Esplanade de la Paix, F-14032 Caen cedex, France. panoff@ibba.unicaen.fr
SL4B. Stabilization of Bacterial Cells for Biosensor Applications R. Pedahzur, Rachel Rosen and Shimshon Belkin Division of Environmental Sciences, the Fredy and Nadine Herrmann Graduate School of Applied Science, The Hebrew University of Jerusalem, Jerusalem 91904, Israel ramip@pob.huji.ac.il
SL5B. Cooling Rate is a Determining Factor of the Viability of Different Types of Cells after Freeze-Thawing F. Dumont, P.A. Marechal and P. Gervais Laboratoire de Génie des Procédés Alimentaires et Biotechnologiques ENSBANA 1, Esplanade Érasme, F-21000 Dijon, France dumontf@u-bourgogne.fr
SL6B. Stabilization of Yeast Cells during Freezing: Interaction Between Temperature Level and Temperature Kinetics P.A. Marechal, F. Dumont and P. Gervais ENSBANA, University of Burgundy, 1 esplanade Erasme 21000 Dijon. France marechal@u-bourgogne.fr
SL7B. Novel Adaptive Response to Near-Freezing Temperaures in Yeast Olga Kandror, Nancy Bretschneider, Duccio Cavalieri, Alfred L. Goldberg Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA; Bauer Center for Genomic Research, Harvard University, Cambridge, MA 02138, USA okandror@hms.harvard.edu
TUESDAY
9:00 A. Cryopreservation Solutions and Protocols
SL8A. Isothermal Drying Kinetics of Carbohydrate Solutions Alptekin Aksan and Mehmet Toner Center for Engineering in Medicine, Harvard Medical School, Department of Surgical Services, Massachusetts General Hospital, The Shriners Burns Hospital, Boston, MA 02114, USA mtoner@sbi.org
SL9A. Cryopreservation, Hepatocyte Monolayers, and DMSO Shock D. Stevenson, M.H. Grant, E. Goldie, G. Connel and C Morgan Bioengineering Unit, Strathclyde University, Glasgow, Scotland G4 0NW, United Kingdom
SL10A. Theoretical Prediction of ‘Optimal’ Freezing Programmes H. Woelders and A.E.N. Chaveiro Animal Sciences Group, Wageningen UR, Division of Animal Resources Development P.O. Box 65, 8200 AB, Lelystad, The Netherlands henri.woelders@wur.nl
SL11A. Optimization of Cryopreservation Protocols using Computer Simulations L.E. McGann and J.A.W. Elliott Department of Laboratory Medicine and Pathology, Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta T6G 2R8, Canada locksley.mcgann@ualberta.ca
B. Low Temperature Chemistry and Physics of Biologically Relevant Molecules
SL8B. Vibrational Properties of Cryoprotectants in Water Mixtures by Inelastic Neutron Scattering S. Maccarrone, A. Mangione, S. Magazù and F. Migliardo Department of Physics, University of Messina, I-98166 Messina, Italy fmigliar@dsme01.unime.it
SL9B. Low Temperature Matrix-Isolation and Solid State FT-IR Spectra of N-Methylated Aminoacids: May the Neutral Forms Exist in Solid State? A. Gómez-Zavaglia and R. Fausto Department of Chemistry, University of Coimbra, P-3004-535, Portugal; Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, RA-1113, Argentina angoza@qui.uc.pt
SL10B. Controlled Nucleation in Freezing Biological Material A. Petersen, B. Glasmacher and G. Rau Institute for Biomedical Technologies, RWTH Aachen, D-52074 Aachen (Germany)
SL11B. Results of The Application of An Ultrasound Field on the Statistical Properties of Water Freezing Roberto Baena and Ramon Risco CryoBioTech. Escuela Superior de Ingenieros, Universidad de Sevilla, Spain ramon@us.es
15:30 A. Effects of Freezing and Drying in Cells
SL12A. Membrane Integrity of Desiccated Rat Mitochondria Xiang-Hong Liu and Mehmet Toner Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts, USA x_liu@hms.harvard.edu
SL13A. Effect of Cryopreservation on Mitochondrial DNA of Zebrafish (Danio rerio) Blastomere Cells J. Kopeika, T. Zhang, D. M. Rawson and G. Elgar Luton Institute of Research in the Applied Natural Sciences, University of Luton, The Spires, 2 Adelaide Street, Luton, LU1 5DU, United Kingdom; Human Genome Mapping Project Resource Centre, Cambridge, United Kingdom tiantian.zhang@luton.ac.uk
SL14A. Rapid Loading of Trehalose Induced in J774 Mouse Macrophage Cells Gloria Elliott, Jennifer Cusick, Michael Menze, Trudy Witt, S. Hand and Mehmet Toner Massachusetts General Hospital and Harvard Medical School, Boston, MA, 02114, USA; Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA gelliott@sbi.org
SL15A. Post-Thaw Assessment of Intracellular Ice: Preparation and Experimental Significance Ioana M Croteau and Jason P. Acker Canadian Blood Services; Department of Laboratory Medicine and Pathology, University of Alberta, T6g 2r8 Edmonton, Canadaont> Jason.Acker@ualberta.ca
SL16A. Proteomic Profiling Following Cryopreservation: Evidence for A Complex Set of Biomolecular Events Influencing Cell Survival Martin J. Vogel, John M. Baust, Robert Van Buskirk and John G. Baust Institute of Biomedical Technology, State University of New York, Binghamton, NY 13902-6000, USA mvogel1@binghamton.edu
SL17A. Stability of Thawed Red Cells. A Comparison Between the Low and High Glycerol Method Charles C. M. Lelkens, Femke Noorman, Jack G. Koning, Rosa Truijens-de Lange, Arthur J. Verhoeven and Johan W M Lagerberg Military Blood Bank, Leiden and Sanquin Research at CLB, Laboratory of Cryobiology, Amsterdam, The Netherlands j.lagerberg@sanquin.nl
SL18A. Cryopreservation Response of Osteoblasts Attached to Hydroxyapatite as a Function of Minimum Temperature J. J. McGrath and B. L. Liu Aerospace and Mechanical Engineering Department University of Arizona, Tucson, AZ, 85721, USA mcgrath@ame.arizona.edu
SL19A. Calpain Activation Following Cryopreservation: A Downstream Effector of Cellular Demise Anthony T. Robilotto, John M. Baust, Robert G.Van Buskirk and John G. Baust Institute of Biomedical Technology, State University of New York Binghamton, NY 13902-6000, USA arobilo1@binghamton.edu
SL19'A. Stabilization of Nucleated Mammalian Cells by Drying at Reduced and Low Water Content I. I. Katkov, S. R. Oppenheimer, I. Puhlev, A. M. Mackay and F. Levine UCSD Cancer Center, University of California San Diego, La Jolla, California 92093, USA; Osiris Therapeutics, Inc., Baltimore, Maryland 21231, USA
B. Organs and Tissues Cryoprotection
SL12B. Utilization of Directional Freezing to Construct Tissue Engineering Scaffolds J. Preciado, S. Cohen and B. Rubinsky University of California at Berkeley, USA; Ben Gurion University, Israel preciado@me.berkeley.edu
SL13B. Vitreous Cryopreservation of Tissue-Engineered Constructs L. L. Kuleshova, X. W. Wang, A.Chua, Y. Wu and H. Yu Department of Physiology, Faculty of Medicine, National University of Singapore, 2 Medical Drive, Singapore; Institute of Bioengineering and Nanotechnology, Singapore phskl@nus.edu.sg
SL14B. Post Thaw Function of Cryopreserved Hepatocyte Aggregates A. Hubel and T. B. Darr Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455 USA hubel001@umn.edu
SL15B. Renal Preservation with Sucrose-Based (Sbs) Solution B. Fuller, C. Shurey, A. Petrenko, N. Lane and C. Green Royal Free & University College, Medical School, London NW3 2QG, United Kingdom; Northwick Park Institute for Medical Research, Harrow HA1 3UJ, United Kingdom; Institute for Problems of Cryobiology and Cryomedicine, Kharkov, Ukraine. b.fuller@rfc.ucl.ac.uk
SL16B. The Liver Energetic State After Cold Storage in Uw and Sucrose-Based Solution A. Somov, O. Semenchenko, A. Petrenko and B. Fuller Department of Biochemistry, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, 61015 Kharkov, Ukraine; University Department of Surgery, Royal Free & University College Medical School, London NW3 2QG, United Kingdom somov@ukr.net
SL17B. Strategies for Optimization the Survival of Ovarian Blood Vessels After Whole Organ Cryopreservation Z. Gavish and A. Arav Institute of Animal Science, Agricultural Research Organization (ARO). The Volcani Center, Bet Dagan, Israel gav@agri.huji.ac.il
SL18B. Avrami Kinetics of Intercellular Ice Propagation during Freezing of Confluent Tissues M. L. Sumpter, S. L. Stott and J. O. M. Karlsson Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA karlsson@alum.mit.edu
SL19B. Cryopreservation Effect on Thyroid Organ Culture Biochemical Indices N. A. Volkova and T. P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, 23, Pereyaslavskaya str., Kharkov 61015, Ukraine avolkovn@yahoo.com
WEDNESDAY
9:00 A. Cryopreservation of Oocytes and Embryos
SL20A. Evaluating Viability of Fish Oocytes: A First Step Towards Methods for Cryopreservation Esther Lubzens, Irena Pekarsky, Idit Blais and Iris Meiri National Institute of Oceanography, Haifa; National Center for Mariculture, Eilat, Israel; Oceanographic & Limnological Research, Israel esther@ocean.org.il
SL21A. Studies on Zebrafish (Danio rerio) Oocytes Sensitivity to Chilling and Cryoprotectant Toxicity Tiantian Zhang, Max Plachinta, Anna Isayeva and David Rawson Luton Institute of Research in the Applied Natural Sciences, University of Luton, The Spires, 2 Adelaide Street, Luton, LU1 5DU, United Kingdom tiantian.zhang@luton.ac.uk
SL22A. Progressive Decline of Intracellular Trehalose During Embryonic Development Ali Eroglu, Gloria Elliott, Thomas L. Toth and Mehmet Toner Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia,USA. Surgical Services; Vincent Memorial Obstetrics and Gynecology Service,Massachusetts. General Hospital, Boston, Massachusetts, USA aeroglu@mail.mcg.edu
SL23A. Cryopreservation and Autografting of Ovarian Tissue in a Non-Human Primate A. Hendriama, D. Pawitri, M. Diamente, M. Pangestu and J. Shaw Monash Institute of Reproduction and Development Monash University,Clayton, Vic 3168, Australia; Monash IVF, Clayton Vic 3168 Australia; Centre Primate Research Centre. Bogor Agricultural University, Bogor Indonesia jill.shaw@med.monash.edu.au
B. Cryopreservation of Spermatozoa
SL20B. The Effects of Cryopreservation on Sperm Motility and Vitality C. Miranda, P. Henriques and
T. Almeida Santos
SL21B. Why Can We Vitrify Mammalian Spermatozoa Without Cryoprotectants?: Physical Chemistry Considerations I. I. Katkov, V. Isachenko, E. Isachenko and F. Nawroth UCSD Cancer Center, University of California San Diego, La Jolla, California 92093, USA. Department of Obstetrics and Gynecology, University of Cologne, 50931 Cologne, Germany prodvincell@hotmail.com
SL22B. Advancements in Cryopreservation of Rainbow Trout (Onchorhyncus mykiss) Sperm E. Cabrita, V. Robles and M.P. Herráez Faculty of Marine Sciencies and Environment, University of Algarve; 8000 Faro, Portugal; Department of Cell Biology, Faculty of Biology, University of León, 24071 León, Spain
SL23B. Linear and Non-Linear Cryopreservation Protocols for Spermatozoa: Comparison of Ideal and Non-Ideal Solution Models. Jaime Saenz and Ramon Risco CryoBioTech. Escuela Superior de Ingenieros. Universidad de Sevilla, 41092 Sevilla, Spain. ramon@us.es
THURSDAY
9:00 A. Cryosurgery and Cryomedicine
SL24A. Pre-Existing Inflammation Augments Cryo-Injury in vivo Bo H. Chao and John C. Bischof Departments of Biomedical Engineering, Mechanical Engineering, and Urologic Surgery, University of Minnesota, Minneapolis, MN 55455, USA bischof@umn.edu
SL25A. Stochastic Simulation of The Kinetics of Intercellular Ice Propagation During Cryosurgery S. L. Stott and J. O. M. Karlsson Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA karlsson@alum.mit.edu
SL26A. Cardiomyocyte Responses to Thermal Excursions: Implications for Electrophysiological Cardiac Mapping Kristi K. Snyder, John M. Baust, Robert Van Buskirk and John G. Baust Institute of Biomedical Technology, Binghamton University, Binghamton, NY 13902 USA Bh09474@binghamton.edu
SL27A. In Vitro Evaluation of Thermal and Mechanical Effects of Cryoplasty Erin D. Grassl and John C. Bischof Department of Mechanical Engineering, University of Minnesota, Minneapolis, Minnesota, USA bischof@umn.edu
B. Cryopreservation Procedures
SL24B. Using Simulations to Design a Cryopreservation Procedure for Hematopoietic Stem Cells Without DMSO L. U. Ross-Rodriguez, H. Yang, J. A. W. Elliott and L. E. McGann Canadian Blood Services; Department of Laboratory Medicine and Pathology, Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada ross@ualberta.ca
SL25B. Quantitative Examination of Concentration Change in the Perfusion Chamber Hiroshi Takamatsu and Yuichi Komori Institute for Materials Chemistry and Engineering, Kyushu University, 6-1 Kasugakoen, Kasuga, Japan; Interdisciplinary Graduate School of Science and Engineering, Kyushu University, Kasuga, Japan takamatu@cm.kyushu-u.ac.jp
SL26B. A Comparison of Various Models of Osmotic Transport H. Y. Elmoazzen, J. A. W. Elliott and L. E. McGann Department of Laboratory Medicine and Pathology, Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada helmoazz@ualberta.ca
SL27B. Use of Alginate and Sugar Solutions for The Improvement of Performances of Lactic Acid Bacteria Subjected to Freezing and Freeze Drying B. De Giulio, R. Coppola, P. Orlando, M. De Rosa, A. Sada, P. P. De Prisco and F. Nazzaro Institute of Food Science and Technology-CNR, Via Roma 52, 83100 Avellino, Italy; Di. S.T.A.A.M. - University of Molise, Via De Sanctis, 86100, Campobasso, Italy; Institute of Protein Biochemistry and Enzymology-CNR, 80100, Napoli, Italy; Dept of Exp. Medic.-II University of Naples, Via Costantinopoli 16, 80100 Napoli, Italy. mena@isa.cnr.it
15:30 A. Low Temperature Storage of Human Embryos
SL28. Successful Vitrification of Human Embryos Using Modified Straw-in-Straw Method L. L. Kuleshova, N. Takeda and Y. Odawara Department of Physiology, Faculty of Medicine, National University of Singapore 2 Medical Drive, Singapore; Odawara Women’s Clinic Reproduction Centre, 2-11-16 Ebisu-Nishi, Shibuya-ku, Tokyo 150-0021, Japan phskl@nus.edu.sg
SL29. Hypothermic Storage of Human Oocytes and Embryos V. I. Grischenko, N. N. Chub and L.V. Kovalinskaya Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua |
CONTACT
CRYOBIOMOL SECRETARIAT
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