POSTERS
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Life at Low Temperatures
P1.1 - Lipid Peroxidation and Catalase Activity in the Cold Tolerant Beetle A. K. Gulevsky, V.V. Ryazantsev, Ye.A. Grischenkova and Li. I. Relina Department of Biochemistry of Cold Adaptation, Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Pereyaslavskaya st. 23, 61015 Kharkov, Ukraine vvr@ticom.kharkov.ua
P1.2 - Electrogenesis of Cooled Brain during Hypothermia and Hibernation G. A Babijchuk, V. S. Marchenko and V. S. Babijchuk Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P1.3 - Protein Synthesis and Degradation in Hibernators G. F. Zhegunov and G. P. Zhegunova Kharkov State Veterinary Academy, Kharkov, Ukraine Zhegunov@ukr.net
Cryopreservation of Plants and Vegetal Cells
P2.1 - Survival and Genetic Stability of Chrysanthemum Morifolium Shoot Apices After Cryopreservation by Vitrification or Encapsulation-Dehydration M. E. González-Benito and C. Martín Departamento de Biología Vegetal, Universidad Politécnica de Madrid, 28040 Madrid, Spain egonzalez@agricolas.upm.es
P2.2 - Shoot Tip Cryopreservation of two Andean Tubers: Bitter Potato (Solanum Juzepczukii Bulk.) and Oca (Oxalis Tuberosa Mol.) V. H. Mendoza Condori, M. E. González-Benito, A. D. Molina-García and C. Pérez Dpto. de Biología Vegetal, Universidad Politécnica de Madrid, Ciudad Universitaria Madrid; Instituto del Frío, Consejo Superior de Investigaciones Científicas, Madrid
P2.3 - Protection During Freezing of Pea Thylakoid Membranes With Altered Membrane Fluidity A. V. Popova and M. Y. Velitchkova Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G. Bonchev str. Bl. 21, 1113 Sofia, Bulgaria mayav@obzor.bio21.bas.bg
P2.4 - Cryopreservation of Fruit Trees by Encapsulation-Dehydration Method A. Bilavcik and J. Zamecnik Department of Molecular Biology, Research Institute of Crop Production, Drnovska 507, 16106 Prague 6, Czech Republic bilavcik@vurv.cz
P2.5 - Comparison of Encapsulation-Dehydration and Ultra-Rapid Freezing Method for Potatoes M. Faltus, J. Zámečník and M. Grospietsch Department of Molecular Biology, Research Institute of Crop Production, Drnovská 507, CZ-16106 Prague 6, Czech Republic faltus@vurv.cz
Mechanisms of Adaptation of Microorganisms to Low Temperature and Cryopreservation of Microorganisms
P3.1 - Flow Cytometric Assessment of Membrane Damage During Freezing, Freeze-Drying and Storage of Lactobacillus acidohilus Rikke Stavnsbjerg, Inge Knap and Jakob Worm Process Development, Rda, Chr. Hansen a/s, Hoersholm, Denmark rikke.stavnsbjerg@dk.chr-hansen.com
P3.2 - Sterility Determination for Crypreserved Fetoplacental Preparations A.S. Snurnikov, N. G. Kadnikova and V. F. Martsenyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P3.3 - Effect of Cyclic Change in Storage Temperature on Viability of Cryopreserved Cells of Pro- and Eukaryotes I. P. Vysekantsev, T. F. Petrenko, E. I. Goncharuk, S. V. Koschij, E. V. Kudokotseva, V. F. Martsenyuk and T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of The National Academy of Sciences of The Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P3.4 - Natural Sellection Increases Cryotolerance of Lactobacillus delbrueckii subsp. bulgaricus C. Beal, C. Monnet and G. Currieu UMR Microbiology of Food Procedures, Thiverval Grignon, France beal@grignon.inra.fr
Cryopreservation Solutions and Cryoprotection Procedures and Protocols
P4.1 - Thermal Properties of Ethanediol Regarding Vitrification V. Odagescu, A. Baudot, and P. Boutron CRTBT, CNRS, BP 166, 38042 Grenoble Cedex 9, France; Laboratoire Louis Neel, CNRS, BP 166, 38042 Grenoble Cedex 9, France baudot@grenoble.crns.fr
P4.2 - Mechanism of Cytotoxic and Cryoprotective Effect of Penetrating Cryoprotectants of Amide and Diol Series T. P. Linnik, A. M. Kompaniets and A. V. Nikolenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P4.3 - Cryorenewal is the Base for the Progress of Current Cryobiological TechnologiesV. I. Grischenko and E. I. Alekseyevskaya Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
Low Temperature Chemistry and Physics of biologically Relevant Molecules
P5.1 - Estimation of the Contribution of Intramolecular H-bonding Entropy to the Conformational Stability of Glycine and Alanine Conformations by Matrix Isolation FT-IR Spectroscopy B. Lambie, R. Ramaekers, J. Pajak and G. Maes Department of Chemistry, University of Leuven, B-3001 Leuven, Belgium; Faculty of Chemistry, University of Wroclaw, 50-383 Wroclaw, Poland bert.lambie@chem.kuleuven.ac.be
P5.2 - Conjugated Ketene, Dewar Valence Isomer and Cyclobutadiene Photochemically Generated From a-Pyrone and 4,6-Dimethyl-a-Pyrone. A Matrix Isolation Study S. Breda, L. Lapinski, I. Reva and R. Fausto Department of Chemistry, University of Coimbra, Coimbra, P-3004-535, Portugal; Institute of Physics, Polish Academy of Sciences, Warsaw, PL-02-668, Poland sbreda@qui.uc.pt
P5.3 - Photoisomerization of Matrix Isolated 4-Methoxy- and 4-Hydroxy-6-Methyl-a-Pyrones S. Breda, L. Lapinski, M.. Nowak and R. Fausto Department of Chemistry, University of Coimbra, Coimbra, P-3004-535, Portugal;Institute of Physics, Polish Academy of Sciences, Warsaw, PL-02-668, Poland sbreda@qui.uc.pt
P5.4 - Applications of Novel Low Temperature Mass Spectrometric Technique in Molecular Cryobiophysics M. V. Kosevich, V. S. Shelkovsky and O. A. Boryak Department of Molecular Biophysics, B. Verkin Institute for Low Temperature Physics and Engineering of NAS Ukraine, 47, Lenin Avenue, Kharkov, Ukraine mvkosevich@ilt.kharkov.ua
P5.5 - Infrared Matrix-Isolation Study of Dithiooxamide: Ground Conformational State Characterization M. Yaman, L. Lapinski, I. Reva, R. Fausto and C. Öğretir Department of Chemistry, University of Coimbra,30004-535 Coimbra, Portugal; Institute of Physics, Polish Academy of Sciences, 02-668 Warsaw, Poland; Department of Chemistry, University of Osmangazi, 26480 Eskişehir, Turkey mujganyaman@yahoo.com
P5.6 - Theoretical Study of the Conformations, Vibrational and Electronic Spectra of Methacryloyl Halides Archa Sharma,V.P Gupta and Ajit Virdi Department of Physics, University of Janmmu, Jammu-Tawi 180 006, India Ar_sh1@Rediffmail.com
P5.7 - Quantum Chemical Study on Rotational Isomerism and Vibrational and Electronic Spectra of Some a,b-Unsaturated Esters Ajit Virdi, V.P. Gupta and Archana Sharma Department of Physics, University of Jammu, Jammu-Tawi 180 006, India virdi_123@indiatimes.com
P5.8 - A Combined Matrix-Isolation Spectroscopy and Theoretical Study of Serine Neutral Form S. Jarmelo, L. Lapinski, and R. Fausto Department of Chemistry, University of Coimbra,30004-535 Coimbra, Portugal; Institute of Physics, Polish Academy of Sciences, 02-668 Warsaw, Poland sjarmelo@qui.uc.pt
Effects of Freezing and Drying in Cells
P6.1 - The Effect of Cell-Cell and Cell-Matrix Interactions on Freezing Response: a Role for Cell Micropatterning? Jason P. Acker and Kenneth Wong Canadian Blood Services; Department of Laboratory Medicine and Pathology, University of Alberta, T6G 2R8 Edmonton, Canada Jason.Acker@ualberta.ca
P6.2 - Cryopreservation of Erythrocytes Derived from Human Cord Blood L. A. Babijchuk, N. G. Zemlyanskikh and E. S. Korzh Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P6.3 - Investigation of Rapid Freezing and Cooling Rates of Cells in Cylindrical Containers with Cryoprotective Media V. I. Grischenko, V. I. Babenko and A.V. Dunayevskaya Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, 23 Pereyaslavskaya str., Kharkov 61015, Ukraine;Institute of Low Temperature Physics and Engineering, 47 Lenin’s Ave, Kharkov, 61103, Ukraine Dunayevskaua@yahoo.co.uk
P6.4 - Cryopreservation Effect on Morphological Indices of Adrenal Culture A.V. Gerashchenko Institute for Problems of Endocrine Pathology named by V.Ya. Danilevsky, Kharkov, Ukraine geranru@yahoo.com
P6.5 - Activation of Caspase-2 and Caspase-3 Following Cryopreservation Eric R. James, Lorie L. Robertson, Lia Campbell and Michael G. Taylor Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA and Organ Recovery Systems Inc., Charleston, SC. USA jameser@musc.edu
P6.5' - Thermogravimetrical Determination of Water Content of Cells Dried in Monolayers: Advantages and Pitfalls I. I. Katkov and F. Levine UCSD Cancer Center, University of California San Diego, La Jolla, California 92093, USA prodvincell@hotmail.com
P6.6 - Effect of Various pH Solutions on Structural and Functional Properties of Rat Myelokaryocytes in the Norm and During Autoimmune Pathology Yu. A. Kozlova, T. G. Dubrava, N. N. Babenko, M. V. Ostankov, E. D. Lutsenko and A. N. Goltsev Cryopathophysiology Department, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P6.7 - Regulation of Rat’s Hepatocyte Cell Volume Under Non-Isotonic Conditions L.G. Kuleshova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P6.8 - Direct Measurement of Size Change in Cells Subjected to Osmotic Stress Andres Lobeiras Beckman Coulter Inc. Particle Characterization Group 11800 S.W. 147th Ave. M/C 32-B13 Miami, FL 33196, USA andres.lobeiras@coulter.com
P6.9 - Study of Low Temperature Effect on Rat Liver Microsomes Using the Spectrofluorimetry E. Onischenko, T. S. Dyubko and A. V. Zinchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, 23, Pereyaslavskaya str., Kharkov 61015, Ukraine cryo@online.kharkov.ua
P6.10 - Improvement of Some Cryopreservation Stages of Suspensions Enriched With Stem Haemopoietic Cells A.Yu. Petrenko, Yu. A. Petrenko and N.G. Skorobogatova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine pay@kharkov.ua
P6.11 - Effects of Saccharide on Living Cells during the Freezing-Thawing Process and Effects of their Uptake by Electroporation Ryo Shirakashi, Kilian J. Müller, Vladimir L. Sukhorukov, Ichiro Tanasawa and Ulrich Zimmermann Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505 Japan; Department of Engineering, Nihon University, 1 Nakagawara, Tamura-cho, Koriyama 963-8642, JapanLehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany aa21150@iis.u-tokyo.ac.jp
P6.12 - Successful Cryopreservation of Mammalian Cells With Non-Metabolizable Glucose Compounds K. Sugimachi and M. Toner The Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, MA 02114, USA ksugimachi@hms.harvard.edu
P6.13 - Dynamical Transition in Cryoprotective Mixtures M. Vadalà, A. Mangione, S. Magazù and F. Migliardo Department of Physics, University of Messina, I-98166 Messina, Italy fmigliar@dsme01.unime.it
P6.14 - Involvement of Mitochodrial-Associated Pathway of Apoptosis in Cryopreservation of Normal Human Dermal Fibroblasts Martin J. Vogel, John M. Baust, Robert Van Buskirk and John G. Baust Institute of Biomedical Technology, State University of New York, Binghamton, NY 13902-6000, USA mvogel1@binghamton.edu
P6.15 - Low-Temperature Phase Transition and Glass Transition in Cord Blood Erythrocytes Suspensions in Dependence on 1,2-Propanediol and Glycerol Concentration A. V. Zinchenko and E. N. Bobrova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine cry@online.kharkov.ua
Cryoprotection of Organs and Tissues
P7.1 - Osmotic Changes in Kidnesy during Cryoprotectant Washout W. De Loecker, N Cheng and F. Penninckx Departments of Abdominal Surgery and Biochemistry, Faculteit Geneeskunde, Katholieke Universiteit te Leuven, B-3000, Leuven, Belgium
P7.2 - Biologically Active Substance Extracting from Xenogenic Organs with Applying Cryobiological Technologies S. E. Galchenko, I. V. Belochkina, I. V. Sleta, E. S. Galchenko, N. Yu. Shkodovskaya and B. P. Sandomirsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, 23, Pereyaslavskaya str., Kharkov, 61015 cryo@online.kharkov.ua
P7.3 - Thermodynamics of Cryoprotectant Equilibration in Tissues J. A. W. Elliott, H. Y. Elmoazzen, R. C. Bannerman and L. E. McGann Department of Chemical and Materials Engineering, Department of Laboratory Medicine & Pathology, University of Alberta, Edmonton, Alberta, Canada helmoazz@ualberta.ca
P7.4 - Three-Dimensional Microscopic Behavior of Ice Crystals and Cells During Directional Solidification of Muscle Tissues Hiroshi Ishiguro, Akinobu Kataori and Takashi Horimizu Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka 808-0196, Japan; Graduate School of Engineering Mechanics, University of Tsukuba, Ibaraki 305-8573, Japan ishiguro@life.kyutech.ac.jp
P7.5 - Real Transient Temperature and Mass Fields of Perfused Tissues and Organs from a Digital Image and Finite-Difference Method J. Aguilar, S. Álvarez, J. M. Salmerón and R. Risco CryoBioTech, Escuela Superior de Ingenieros, Universidad de Sevilla, 41092 Sevilla, Spain; Depto. de Ingeniería Energética y Mecánica de Fluidos; Escuela Superior Ingenieros, Universidad de Sevilla, 41092 Sevilla, Spain ramon@us.es
P7.6 - Ultrastructure of Endotheliocytes of Liver Sinusoids Under the Effect of General Cooling of RatsA. S. Kaprelyants, L. N. Marchenko and R. K. Migunova Department of Cryocytology and Quantitative Morphology, Institute for Problems of Cryobiology & Cryomedicine of The National Academy of Sciences of Ukraine, Kharkov, Ukraine kaprelyants@yahoo.com
Cryopreservation of Oocytes and Embryos
P8.1 - DMSO Effect on Rate of Embryo Development in Carp V. V. Cherepanov and E. Ye. Nipot Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, 61015, Ukraine cherepanov@ukr.net
P8.2 - Feasibility of Fish Spermatozoa and Embryo Cryopreservation at High and Super-High Heat Exchange Rates L. V. Gorbunov, N. V. Grinzhevsky, I. A. Morozova and A. V. Medvedovsky Kharkov Biotechnological Center, Kharkov. Fishery Institute, Ukrainian Academy of Agricultural Sciences, Kiev, Ukraine cryo@animal.kharkov.ua
P8.3 - Cytogenetic Analysis of Cryopreserved Human Embryos M. P. Petrushko, V. I. Grischenko, N. N. Erogova and V. I. Pinyaev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, 23, Pereyaslavskaya str., Kharkov 61015, Ukraine cryo@online.kharkov.ua
P8.4 - Cryopreserved Cord Blood Serum to Optimize Reproductive Technologies O. S. Prokopyuk, O. V. Lipina, I. B. Musatova, V. Yu. Prokopyuk Institute for Problems of Cryobiology & Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov Medical State University, Kharkov, Ukraine cryo@online.kharkov.ua
P8.5 - Attempts to Dechorionize Turbot Embryos as a Previous Step for Vitrification V. Robles, E. Cabrita, S. Cuñado, M.P. Herráez Dept. Cell Biology, Faculty of Biology, University of Leon, 24071 Leon, Spain dbcmho@unileon.es
P8.6 - The Role of Frozen Pronuclear Oocyte in the Management of Infertility T. Almeida Santos, S. Figueiredo, R. Brito and P. Henriques Departamento de Medicina Materno-Fetal, Genética e Reprodução Humana. Hospitais da Universidade de Coimbra. Portugal. Faculdade de Medicina da Universidade de Coimbra. Portugal raquelcbrito@hotmail.com
P8.7 - High Ice Nucleation Temperature of Zebrafish Embryos M. Hagedorn, A. Peterson, P. Mazur and F.W. Kleinhans DDepartment of Physics (LD154), Indiana University Purdue University Indianapolis (IUPUI), Indianapolis, USA fkleinha@iupui.edu
Cryopreservation of Spermatozoa
P9.1 - Male Differences in the Sperm Membrane Permeability for Water and Glycerol A. E. N. Chaveiro, J. Liu, J. K. Critser, C. A. Zuidberg and H. Woelders Animal Sciences Group, Wageningen UR, Division Animal Resources Development, P.O. Box 65, 8200 AB, Lelystad, The Netherlands; Comparative Medicine Center, College of Veterinary Medicine, University of Missouri. 1600 East Rollins Road, E-109, Columbia, MO 65211, USA Antonio.chaveiro@wur.nl
P9.2 - Cryopreservation of Cynomolgus Monkey (Macaca fascicularis) Spermatozoa in a Chemically Defined Freezing Medium Yahui Li, Kejun Cai, Lei Su, Mo Guan, Xiechao He and Weizhi Ji Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming, Yunnan, China. Graduate School, The Chinese Academy of Sciences, Beijing, China; College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan, China. wji@mail.kiz.ac.cn
P9.3 - Following Freeze-Thaw Sperm Cell Membrane Integrity and Osmotic Resistance is Greater at Lower Dilution Rates but Not Following Incubation at Room Temperature N. S. Prathalingam, W. V, Holt, S. G. Revell, S. Jones and P. F. Watson Royal Veterinary College, Camden Town, London, NW1 0TU, United Kingdom; Institute of Zoology, Regents Park, London; NW1 4RY, United Kingdom; Genus Breeding Ltd. Ruthin, Denbigshire, LL15 2UP, United Kingdom nprathalingam@rvc.ac.uk
P9.4 - Vitrification of Human Sperm Without Cryoprotectants: Experimental Data V. Isachenko, I.I. Katkov, E. Isachenko and F. Nawroth UCSD Cancer Center, University of California San Diego, La Jolla, California 92093, USA; Department of Obstetrics and Gynecology, University of Cologne, Germany prodvincell@hotmail.com
Cryosurgery and Cryomedicine
P10.1 - Fetal Cells for Treatment of Age-Related Macular Degeneration Yu. A. Demin, L.V. Litvinova and E.I. Goncharuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P10.2 - “Plateks” Application in Treatment of Cornea Dystrophic Diseases Yu. A. Demin, T. M. Sharlay and A.V. Pivnenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine cryo@online.kharkov.ua
P10.3 - Application of Cryopreserved Amniotic Cover at Eye Surface Pathology I. L. Lymar, Yu. A. Demin, V. I. Grischenko and V. I. Strona Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of the Ukraine, Kharkov, Ukraine cryo@online.kharkov.ua
P10.4 - Assessment of Function and Metabolic Activity of Neonatal Cardiomyocytes Following Hypothermic Storage Kristi K. Snyder, John M. Baust, Robert Van Buskirk and John G. Baust Institute of Biomedical Technology, Binghamton University, Binghamton, NY 13902, USA Bh09474@binghamton.edu
Last Minute Confirmation
P11.1 - Temperature Dependence of Tissue Electrical Properties and How it Affects Electrical Impedance Tomography of Cryosurgery J. Edd, L.. Horowitz and B. Rubinsky Dept of Mechanical Engeneering, University of California at Berkeley, CA 94720, USA jonedd@me.berkeley.edu
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CRYOBIOMOL SECRETARIAT
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